Gfp Stable Cell Line

In the studies of life science and biotechnology the commonly used reporter genes are visually identifiable including green fluorescent protein gfp red fluorescent protein rfp luciferase.

Gfp stable cell line. As useful tools for scientific research reporter stable cell lines are those cells involved in expression of reporter protein s which are engineered through integrating the reporter expression cassettes into cell genomes mediated by plasmid or lentivirus vectors. Selection markers can be delivered using the same plasmid that contains the gene of interest in cis or on a separate plasmid in trans that needs to be co transfected with the plasmid containing the gene of interest. 4t1 gfp cmv stable cells t4 a mouse mammary gland tumor cell line has been widely used in cancer research and drug development in vitro and in vivo. Description transformed from sh sy5y cell line originated from a human bone marrow with neuroblastoma by lentivirus transduction this cell line express enhance egfp fluorescent marker.

I am trying to transfect thp 1 cells with gfp lc3 to create a stably expressing cell line. The green fluorescent protein gfp is a protein composed of 238 amino acid residues 26 9kda that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Gfp reporter stable cell line hek293. Stable cell line generation is made possible by the use of positive selection markers such as hygromycin g418 geneticin zeocin and blasticidin antibiotic resistance.

However i can not get a very high transfection efficiency 10 and this expression disappears after 48hrs. Cell line contains a puromycin antibiotic selection marker.